First Time User? Enroll now.
*Vaccine availability and appointments* | Additional COVID-19 resources.
Please be aware that mask-wearing is required at all UNC Health facilities.
Home > Health Library > Overnight Dexamethasone Suppression Test
The overnight dexamethasone suppression test checks to see how taking a steroid medicine called dexamethasone changes the levels of the hormone cortisol in the blood. This test checks for a condition in which large amounts of cortisol are produced by the adrenal glands (Cushing's syndrome).
Normally, when the pituitary gland makes less adrenocorticotropic hormone (ACTH), the adrenal glands make less cortisol. Dexamethasone, which is like cortisol, lowers the amount of ACTH released by the pituitary gland. This in turn lowers the amount of cortisol released by the adrenal glands.
After a dose of dexamethasone, cortisol levels often stay very high in people who have Cushing's syndrome. Sometimes other conditions can keep cortisol levels high during this test. Examples include alcohol use disorder, stress, obesity, kidney failure, pregnancy, and uncontrolled diabetes.
The night before the blood test, you will take a dexamethasone pill. The next morning, the cortisol level in your blood will be measured. If your cortisol level stays high, Cushing's syndrome may be the cause.
An ACTH test is sometimes done at the same time as the cortisol test.
This test is done to check for Cushing's syndrome. It is a condition in which large amounts of cortisol are produced by the adrenal glands.
Many medicines can change the results of this test. You may be asked to stop taking some medicines for 24 to 48 hours before your blood is drawn.
The night before the test (usually at 11:00 p.m.), you will swallow a pill that contains 1 milligram (mg) of dexamethasone. Take the pill with milk or an antacid. This can help prevent an upset stomach or heartburn.
The next morning (usually at 8:00 a.m.), you will have a sample of your blood drawn. A health professional uses a needle to take a blood sample, usually from the arm.
Sometimes a more complete dexamethasone suppression test may be done. For this test, you will take up to 8 pills over 2 days. Then the cortisol levels in your blood and urine will be measured.
When a blood sample is taken, you may feel nothing at all from the needle. Or you might feel a quick sting or pinch.
There is very little chance of having a problem from this test. When a blood sample is taken, a small bruise may form at the site.
Test results are usually ready in a few days.
An abnormal test result may mean that more tests are needed. A normal test result means that you do not have Cushing's syndrome. This syndrome can be hard to diagnose. So if the test results aren't clear or if they don't help explain your symptoms, you may be referred to see an endocrinologist.
Each lab has a different range for what's normal. Your lab report should show the range that your lab uses for each test. The normal range is just a guide. Your doctor will also look at your results based on your age, health, and other factors. A value that isn't in the normal range may still be normal for you.
High cortisol levels may be caused by:
Current as of:
December 2, 2020
Author: Healthwise StaffMedical Review: E. Gregory Thompson MD - Internal MedicineKathleen Romito MD - Family MedicineAlan C. Dalkin MD - Endocrinology
Current as of: December 2, 2020
Author: Healthwise Staff
Medical Review:E. Gregory Thompson MD - Internal Medicine & Kathleen Romito MD - Family Medicine & Alan C. Dalkin MD - Endocrinology
To learn more about Healthwise, visit Healthwise.org.
© 1995-2021 Healthwise, Incorporated. Healthwise, Healthwise for every health decision, and the Healthwise logo are trademarks of Healthwise, Incorporated.
Our interactive Decision Points guide you through making key health decisions by combining medical information with your personal information.
You'll find Decision Points to help you answer questions about:
Get started learning more about your health!
Our Interactive Tools can help you make smart decisions for a healthier life. You'll find personal calculators and tools for health and fitness, lifestyle checkups, and pregnancy.
Feeling under the weather?
Use our interactive symptom checker to evaluate your symptoms and determine appropriate action or treatment.